Minimum Information about a Tailoring Enzyme Repository

MITE0000279

MITE0000280

MITE0000281

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Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	Cleavage of BytA to afford 3-NO2-Tyr
Tailoring	Hydrolysis
Database References	No database crosslinks available
Evidence	Inference from genomic data and chemical structure In vitro assay Knock-out studies
References	doi:10.1016/j.chempr.2025.102438

Example 1

Metadata	Description: Cleavage of BytA to afford 3-NO2-Tyr Intermediate: False
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000280
Entry Status	Status: active
Enzyme Name	RufB
Enzyme Description	Aminopeptidase
Database References	UniProtKB: A0A224AVC8 NCBI GenPept: BBA20960.1 MIBiG: BGC0001763
References	doi:10.1016/j.chempr.2025.102438
Comment	RufB is a serine aminopeptidase that cleaves the nitrated BytA pentapeptide
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-04-14)

Contributor(s)	0000-0003-2971-646X
Reviewer(s)	0000-0002-9747-3423 0000-0001-6534-6609
Comment	"New entry. Reviewer 2 added MIBiG crossref and replaced UniProt and GenPept IDs."