MITE0000256
MITE0000257
MITE0000258
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Reaction Information

Number of Known Reactions: 2

Reaction 1
reaction SMARTS
Description Dehydratation of Ser/Thr residues to Dha/Dhb in the biosynthetic pathway of nosiheptide, after NosIJK-catalyzed addition of MIA. Current knowledge indicates that the full leader peptide is required for substrate recognition.
Tailoring
  • Dehydration
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Dehydratation of Ser/Thr residues on nascent NosM precursor peptide.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 2
reaction SMARTS
Description Dehydratation of Ser/Thr residues to Dha/Dhb in the biosynthetic pathway of nosiheptide, without attached MIA heterocycle (in vitro reaction). Current knowledge indicates that the full leader peptide is required for substrate recognition.
Tailoring
  • Dehydration
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Dehydratation of Ser/Thr residues on nascent NosM precursor peptide.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000257
Entry StatusStatus: active
Enzyme NameNosE
Enzyme DescriptionDehydratase
Database References
Cofactors
  • Organic
    • Adenosine triphosphate
  • Inorganic
    • Mg
References
CommentNosE dehydrates Ser/Thr residues together with NosD analogous to the lanthipeptide dehydratase LanB. MIA-addition by NosIJK increases catalytic efficiency but is not essential.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2025-12-30)

Contributor(s)
  • Anonymous MITE contributor
Reviewer(s)
Comment "New entry."

Auxiliary Enzyme #1

Enzyme Name NosD
Description Dehydratase
Database References