Minimum Information about a Tailoring Enzyme Repository

MITE0000248

MITE0000249

MITE0000250

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Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	Cyclization of the ACV substrate to isopenicillin N in the biosynthetic pathway of beta-lactam antibiotics
Tailoring	Cyclization
Database References	RHEA: 22428 BRENDA: 1.21.3.1 Expasy ENZYME: 1.21.3.1
Evidence	Heterologous expression
References	doi:10.1038/ncomms15202 doi:10.1038/nbt0190-39

Example 1

Metadata	Description: Cyclization of δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine (LLD-ACV) tripeptide to isopenicillin N. Intermediate: True
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000249
Entry Status	Status: active
Enzyme Name	PcbC
Enzyme Description	Isopenicillin N synthase
Database References	UniParc: UPI0000168962 NCBI GenPept: ABA70583.1 MIBiG: BGC0000404
Cofactors	Inorganic Fe
References	doi:10.1038/ncomms15202 doi:10.1038/nbt0190-39
Comment	Catalyzes the formation of the beta-lactam ring in the biosynthetic pathway of penicillin and related antibiotics.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-01-01)

Contributor(s)	Anonymous MITE contributor
Reviewer(s)	0000-0003-4819-2251
Comment	"New entry. Added original ref and removed another (not relevant) one. Minor corrections to descriptions."