Minimum Information about a Tailoring Enzyme Repository

MITE0000051

MITE0000052

MITE0000053

Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	thiazole heterocyclisation of Cys8 of bottromycin-precursor
Tailoring	Heterocyclization
Database References	No database crosslinks available
Evidence	Heterologous expression
References	doi:10.1021/jacs.7b09899

Example 1

Metadata	Description: bottromycin A2-precursor peptide with cleaved N-terminal Met Intermediate: True
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000052
Entry Status	Status: active
Enzyme Name	BotC
Enzyme Description	YcaO protein
Database References	UniProtKB: K4MJT7 NCBI GenPept: AFV25482.1 MIBiG: BGC0000469
References	doi:10.1021/jacs.7b09898 doi:10.1039/d0np00097c doi:10.1021/jacs.7b09899
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2024-07-30)

Contributor(s)	0000-0001-6534-6609
Reviewer(s)	0000-0001-6534-6609
Comment	"Initial entry. Reviewer 1 removed parentheses around reaction SMARTS (falsely indicating an intramolecular reaction), reaction passes."

Version 2 (2025-08-26)

Contributor(s)	Anonymous MITE contributor
Reviewer(s)	0000-0001-6534-6609
Comment	"Homogenized enzyme descriptions"