Minimum Information about a Tailoring Enzyme Repository

MITE0000037

MITE0000038

MITE0000039

Reaction Information

Number of Known Reactions: 2

Reaction 1

Description	Introduces the second biaryl-ether crosslink between bht2 and hpg4 in the vancomycin and balhimycin precursor. The reaction occures while the vancomycin precursor is bound to the PCP of module 6 or 7. The enzyme only accepts the chlorinated precursor.
Tailoring	Cyclization Oxidation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1021/acschembio.7b00456

Example 1

Metadata	Description: Introduces the second biaryl-ether crosslink between bht2 and hpg4 in the vancomycin and balhimycin precursor Intermediate: True
Substrate 1
Product 1

Reaction 2

Description	The reaction might be less specific than depicted in the reaction SMARTS. There is no detailed study on the specificity so far. The reaction occurs probably while the precursor peptide backbone is bound to the NRPS as shown for the closely related vancomycin biosynthesis (doi:10.1002/ANIE.200461278).
Tailoring	Cyclization Oxidation
Database References	No database crosslinks available
Evidence	Knock-out studies
References	doi:10.1002/1521-3773(20011217)40:24<4688::AID-ANIE4688>3.0.CO;2-M doi:10.1016/j.jbiotec.2006.04.009

Example 1

Metadata	Description: No description available Intermediate: True
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000038
Entry Status	Status: active
Enzyme Name	OxyA
Enzyme Description	Cytochrome P450
Database References	UniProtKB: F8STX6 NCBI GenPept: AEI58868.1 MIBiG: BGC0000455
Cofactors	Organic Heme Inorganic Fe
References	doi:10.1021/acschembio.7b00456 doi:10.1016/j.jbiotec.2006.04.009 doi:10.1128/AAC.43.7.1565 doi:10.1002/1521-3773(20011217)40:24<4688::AID-ANIE4688>3.0.CO;2-M
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2024-07-30)

Contributor(s)	0009-0008-7609-4484
Reviewer(s)	0000-0001-6534-6609 0000-0003-0443-9902 0000-0002-9747-3423
Comment	"Initial entry. Reviewer 1 partially fixed reactionSMARTS:reactionSMARTS, validation still not passing. Reviewer 1 partially fixed reactions:substrate, validation still not passing. Reviewer 1 partially fixed reactions:products, validation still not passing. Reviewer 2 reworked reaction SMARTS from scratch, reaction passing. Reviewer 2 added information from MITE0000026. Reviewer 3 changed text of enzyme description and enzyme reaction description"

Version 2 (2025-07-25)

Contributor(s)	Anonymous MITE contributor
Reviewer(s)	0000-0001-6534-6609
Comment	"Added cofactor information"

Version 3 (2025-08-13)

Contributor(s)	Anonymous MITE contributor
Reviewer(s)	0000-0001-6534-6609
Comment	"Fixed URL formatting"

Version 4 (2025-08-26)

Contributor(s)	Anonymous MITE contributor
Reviewer(s)	0000-0001-6534-6609
Comment	"Homogenized enzyme descriptions"