Minimum Information about a Tailoring Enzyme Repository

MITE0000036

MITE0000037

MITE0000038

Reaction Information

Number of Known Reactions: 15

Reaction 1

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with UDP-2-fluoro-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 2

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with UDP-6-azido-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 3

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with UDP-2-azido-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 4

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with TDP-4-amino-6-deoxy-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 5

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with UUDP-6-chloro-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 6

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with TDP-4-deoxy-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 7

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with TDP-3-deoxy-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 8

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with TDP-2-deoxy-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 9

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1021/bi010050w doi:10.1186/1471-2164-15-363 doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with TDP-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 10

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with TDP-3-amino-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 11

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with UDP-2-amino-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 12

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with UDP-xylose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 13

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with TDP-6-deoxy-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 14

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with UDP-6-amino-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Reaction 15

Description	Transfers glucose onto hpg4 in the vancomycin and teicoplanin aglycone.
Tailoring	Glycosylation
Database References	No database crosslinks available
Evidence	In vitro assay
References	doi:10.1016/S1074-5521(02)00270-3

Example 1

Metadata	Description: Reaction with TDP-4-amino-glucose and vancomycin intermediate as substrates. Intermediate: True
Substrate
Product 1

Enzyme Information

Accession ID	MITE0000037
Entry Status	Status: active
Enzyme Name	GtfE
Enzyme Description	Glycosyltransferase
Database References	UniProtKB: P96565 NCBI GenPept: AEI58873.1 MIBiG: BGC0000455
References	doi:10.1186/1471-2164-15-363 doi:10.1021/bi010050w doi:10.1016/S1074-5521(02)00270-3
Comment	The enzyme accepts the vancomycin and teicoplanin aglycone as substrate. Therefore, the SMARTS was reduced to the maximum common substructure of both glycopeptide precursors. The true substrate specificity might still be broader.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2024-07-30)

Contributor(s)	0009-0008-7609-4484
Reviewer(s)	0000-0001-6534-6609 0000-0002-9747-3423
Comment	"Initial entry. Reviewer 1 fixed reaction SMARTS and reworked the reactions to take the actual vancomycin precursor instead of a truncated example structure. Reviewer 2 changed text of enzyme description and enzyme reaction description."