Minimum Information about a Tailoring Enzyme Repository

MITE0000314

MITE0000315

MITE0000316

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Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	Oxidation of primaradien/stemoden-oic acid core structure.
Tailoring	Hydroxylation Monooxygenation Oxidation
Database References	BRENDA: 1.14.14.111 Expasy ENZYME: 1.14.14.111
Evidence	Heterologous expression
References	doi:10.1111/j.1365-313X.2010.04408.x

Example 1

Metadata	Description: Oxidation of syn-pimara-7,15-diene to syn-pimara-7,15-dien-19-oic acid. Intermediate: True
Substrate 1
Product 1

Example 2

Metadata	Description: Oxidation of syn-stemod-13(17)-ene to syn-stemod-13(17)-en-19-oic acid Intermediate: True
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000315
Entry Status	Status: active
Enzyme Name	OsCYP99A3
Enzyme Description	9-beta-pimara-7,15-diene oxidase
Database References	UniProtKB: Q0JF01 NCBI GenPept: CAE04106.1
Cofactors	Organic Heme
References	doi:10.1111/j.1365-313X.2010.04408.x
Comment	Oxidation of syn-pimara-7,15-diene to, sequentially, syn-pimara-7,15-dien-19-ol, syn-pimara-7,15-dien-19-al, and syn-pimara-7,15-dien-19-oic acid. The same behavior with syn-stemod-13(17)-ene
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-04-13)

Contributor(s)	0000-0002-7239-6767
Reviewer(s)	0000-0001-5863-7248 0000-0001-6534-6609
Comment	"New entry. Reviewer 1 added "hydroxylation " and "monooxygenation" to tailoring reaction. Reviewer 2 generalized reaction to minimal common substructure and added second example."