Minimum Information about a Tailoring Enzyme Repository

MITE0000297

MITE0000298

MITE0000299

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Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	Hydroxylation of aspergillic acid to hydroxyaspergillic acid
Tailoring	Hydroxylation
Database References	No database crosslinks available
Evidence	Knock-out studies
References	doi:10.1016/j.fgb.2018.04.009

Example 1

Metadata	Description: Aliphatic hydroxylation - Hydroxylation of aspergillic acid to hydroxyaspergillic acid Intermediate: False
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000298
Entry Status	Status: active
Enzyme Name	AsaB
Enzyme Description	Hydroxylase
Database References	UniProtKB: B8N0E7 NCBI GenPept: EED55030.1
Cofactors	Inorganic Fe
References	doi:10.1016/j.fgb.2018.04.009 doi:10.1038/s41598-025-22432-1 doi:10.3389/ffunb.2022.1029195
Comment	Pathway proceeds from deoxyaspergillic acid formed by AsaC, to aspergillic acid via hydroxylation by AsaD, and further to hydroxyaspergillic acid through AsaB. A defining property of aspergillic acid is its ability to chelate ferric iron (Fe3+). See also MIBiG entry BGC0001516)
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-03-25)

Contributor(s)	0000-0003-3141-732X
Reviewer(s)	0000-0002-9747-3423 0000-0001-5863-7248
Comment	"New entry. Reviewer 2 added cofactor information - reaction cannot work without iron."