Minimum Information about a Tailoring Enzyme Repository

MITE0000296

MITE0000297

MITE0000298

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Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	Conversion of deoxyaspergillic acid into aspergillic acid via N-hydroxylation
Tailoring	Hydroxylation
Database References	No database crosslinks available
Evidence	Knock-out studies
References	doi:10.1016/j.fgb.2018.04.009

Example 1

Metadata	Description: Conversion of deoxyaspergillic acid into aspergillic acid via N-hydroxylation Intermediate: True
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000297
Entry Status	Status: active
Enzyme Name	AsaD
Enzyme Description	Cytochrome P450
Database References	UniProtKB: B8N0E9 NCBI GenPept: EED55032.1
Cofactors	Organic Heme Inorganic Fe
References	doi:10.1016/j.fgb.2018.04.009 doi:10.1038/s41598-025-22432-1 doi:10.3389/ffunb.2022.1029195
Comment	Pathway proceeds from deoxyaspergillic acid, formed by AsaC, to aspergillic acid via hydroxylation by AsaD, and further to hydroxyaspergillic acid through AsaB (see also MIBiG entry BGC0001516).
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-03-25)

Contributor(s)	0000-0003-3141-732X
Reviewer(s)	0000-0002-9747-3423
Comment	"New entry."