MITE0000241
MITE0000242
MITE0000243
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Reaction Information

Number of Known Reactions: 4

Reaction 1
reaction SMARTS
Description Hydroxylation of tirandamycin C as first tailoring step in the tirandamycin pathway. TamI oxygenation requires a specific sequence of oxygenations, showing high substrate specificity.
Tailoring
  • Hydroxylation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
  • Site-directed mutagenesis
References
Example 1
Metadata
  • Description: Hydroxylation of tirandamycin C to tirandamycin E.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 2
reaction SMARTS
Description Hydroxylation of tirandamycin E as second tailoring step in the tirandamycin pathway, via the TamL-independent route with spontaneous dehydration.
Tailoring
  • Hydroxylation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Hydroxylation of tirandamycin E to tirandamycin D.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 3
reaction SMARTS
Description Epoxidation of tirandamycin D as third tailoring step in the tirandamycin pathway.
Tailoring
  • Other
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Epoxidation of tirandamycin D to tirandamycin A.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 4
reaction SMARTS
Description Hydroxylation of tirandamycin A as forth tailoring step in the tirandamycin pathway.
Tailoring
  • Hydroxylation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Hydroxylation of tirandamycin A to the mature tirandamycin B.
  • Intermediate: False
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000242
Entry StatusStatus: active
Enzyme NameTamI
Enzyme DescriptionCytochrome P450
Database References
Cofactors
  • Organic
    • Heme
  • Inorganic
    • Fe
References
CommentWorks in tandem with TamL in tirandamycin biosynthesis in a oxygenation-oxidation-oxygenation cascade. However, TamI can also produce mature tirandamycin C, albeit in an less effective fashion.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-01-01)

Contributor(s)
  • Anonymous MITE contributor
Reviewer(s)
Comment "New entry."

Auxiliary Enzyme #1

Enzyme Name TamL
Description Flavoprotein monooxygenase
Database References