MITE0000187

MITE0000188

MITE0000189

Reaction Information

Number of Known Reactions: 5

Reaction 1
reaction SMARTS
Description Oxidation of 12-C hydroxyl group of pre-borrelidin to a carbonyl
Tailoring
  • Oxidation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • Knock-out studies
  • Site-directed mutagenesis
References
Example 1
Metadata
  • Description: No description available
  • Intermediate: True
Substrate substrate SMILES
Product 1 product SMILES
Reaction 2
reaction SMARTS
Description Hydroxylation of 12-C of pre-borrelidin
Tailoring
  • Hydroxylation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • Knock-out studies
  • Site-directed mutagenesis
References
Example 1
Metadata
  • Description: No description available
  • Intermediate: True
Substrate substrate SMILES
Product 1 product SMILES
Reaction 3
reaction SMARTS
Description Di-hydroxylation of 12-C-bound primary amine of pre-borrelidin
Tailoring
  • Hydroxylation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • Knock-out studies
  • Site-directed mutagenesis
References
Example 1
Metadata
  • Description: No description available
  • Intermediate: True
Substrate substrate SMILES
Product 1 product SMILES
Reaction 4
reaction SMARTS
Description Aldoxime-formation via dehydation of N,N-dihydroxylamine
Tailoring
  • Dehydration
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • Knock-out studies
  • Site-directed mutagenesis
References
Example 1
Metadata
  • Description: No description available
  • Intermediate: True
Substrate substrate SMILES
Product 1 product SMILES
Reaction 5
reaction SMARTS
Description Nitrile-formation via dehydation aldoxime
Tailoring
  • Dehydration
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • Knock-out studies
  • Site-directed mutagenesis
References
Example 1
Metadata
  • Description: No description available
  • Intermediate: False
Substrate substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000188
Entry StatusStatus: active
Enzyme NameBorI
Enzyme DescriptionCytochrome P450 monooxygenase
Database References
Cofactors
  • Organic
    • Heme
  • Inorganic
    • Fe
References
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2024-12-01)

Contributor(s)
Reviewer(s)
Comment "New entry. Reviewer 1 added missing reactions for BorI"

Version 2 (2025-07-25)

Contributor(s)
  • Anonymous MITE contributor
Reviewer(s)
Comment "Added cofactor information"

Auxiliary Enzyme #1

Enzyme Name BorK
Description Dehydrogenase, improves conversion rate of BorI
Database References