MITE0000310
MITE0000311
MITE0000312
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Reaction Information

Number of Known Reactions: 2

Reaction 1
reaction SMARTS
Description hydroxylating 3-Me-l-Tyr to 3-OH-5-Me-l-Tyr
Tailoring
  • Monooxygenation
Database References No database crosslinks available
Evidence
  • Knock-out studies
References
Example 1
Metadata
  • Description: Heme-containing peroxygenase; SfmD catalyzes the regioselective hydroxylation of 3-methyl-l-tyrosine (3-Me-l-Tyr) to produce 3-hydroxy-5-methyl-l-tyrosine (3-OH-5-Me-l-Tyr)
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 2
reaction SMARTS
Description Hydroxylating L-Tyr to 3-OH-L-Tyr due to substrate promiscuity
Tailoring
  • Monooxygenation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
References
Example 1
Metadata
  • Description: Converts L-Tyr to 3-OH-L-Tyr in the saframycin A pathway.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000311
Entry StatusStatus: active
Enzyme NameSfmD
Enzyme DescriptionHeme-containing peroxygenase
Database References
Cofactors
  • Organic
    • Ascorbic acid
    • Heme
  • Inorganic
    • Fe
References
CommentSfmD catalyzes the regioselective hydroxylation of 3-methyl-l-tyrosine (3-Me-l-Tyr) to produce 3-hydroxy-5-methyl-l-tyrosine (3-OH-5-Me-l-Tyr). However, it can also hydroxylate L-Tyr.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-04-13)

Contributor(s)
Reviewer(s)
Comment "New entry. Reviewer 2 merged duplicate entry created by Contributor 2."