MITE0000272
MITE0000273
MITE0000274
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Reaction Information

Number of Known Reactions: 1

Reaction 1
reaction SMARTS
Description Bromination of C-8 of W1 of darobactin A, yielding bromodarobactin A.
Tailoring
  • Halogenation
Database References
Evidence
  • Heterologous expression
  • In vitro assay
References
Example 1
Metadata
  • Description: Bromination of darobactin A at the C-8 position of W1, validated by in vitro assay with purified DarHPse, NaBr, FAD, and flavin reductase.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000273
Entry StatusStatus: active
Enzyme NameDarH
Enzyme DescriptionHalogenase
Database References
Cofactors
  • Organic
    • FAD
References
CommentFAD-dependent halogenase responsible for bromination of W1 of darobactin A, yielding bromo-darobactin. Represents a new class of flavin-dependent halogenases with a novel fold, co-purified with FAD.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-03-30)

Contributor(s)
  • Anonymous MITE contributor
Reviewer(s)
Comment "New entry."