Minimum Information about a Tailoring Enzyme Repository

MITE0000207

MITE0000208

MITE0000209

Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	C-terminal cleavage of glycin from lassopeptide
Tailoring	Hydrolysis
Database References	No database crosslinks available
Evidence	Heterologous expression Knock-out studies
References	doi:10.1038/s41586-025-08723-7

Example 1

Metadata	Description: Cleavage of C-terminal glycin cyclicized lariocidin A lassopeptide, resulting in lariocidin C Intermediate: False
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000208
Entry Status	Status: active
Enzyme Name	LrcF
Enzyme Description	Peptidase, clostripain-family
Database References	NCBI GenPept: XMN14533.1
References	doi:10.1038/s41586-025-08723-7
Comment	Plays a role in internal macrocyclization of lariocidin leading to congeners lariocidin B but mechanism is not yet elucidated.
Enzyme Visualization (AlphaFold-predicted)	UniProt ID not available - could not render visualization

Version 1 (2025-03-27)

Contributor(s)	0000-0001-6534-6609
Reviewer(s)	0000-0003-4819-2251
Comment	"Initial entry. Reviewer 1 fixed smiles and smarts (corrected lacton with lactam)"